Академический Документы
Профессиональный Документы
Культура Документы
中研院基 因體 研究中 心 助研 究員
台大分 子醫 學研究 所兼 任助 理教授
Functional Genomics
中研 院基 因體研 究中 心
Tel: (02) 2789-9930 ext: 253 (O); 2789-8775 (L)
ljjuan@gate.sinica.edu.tw
Gene Expression
Virus-Cell Interaction
Virus-Cell Interaction
Could chromatin modifiers be the
common targets?
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Alberts et al
Nature 1997 389, 251
Cell Dec 2005
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Nature
(2003)
424, 147
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold
Histone modifiers
ATP-dependent chromatin remodeling complexes
DNA demethylases
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold
Histone modifiers
ATP-dependent chromatin remodeling complexes
DNA demethylases
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold
Histone modifiers
ATP-dependent chromatin remodeling complexes
DNA demethylases
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold
Histone modifiers
ATP-dependent chromatin remodeling complexes
DNA demethylases ?
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Repressors Activators
Corepressors Coactivators
Scaffold Scaffold
Histone modifiers Histone modifiers
ATP complex ATP-dependent chromatin remodeling complexes
DNA methylases DNA demethylases ?
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold
Histone modifiers
ATP-dependent chromatin remodeling complexes
DNA demethylases
Active chromatin
Santos-Rosa and Caldas, Euro J. Cancer (20
Amino Acid
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold
Histone modifiers
ATP-dependent chromatin remodeling complexes
DNA demethylasesHistone acetyltransferase (HAT)/
Histone deacetylases (HDAC)
K-Ac
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Histone Coactivators
methyltransferase Scaffold
(HMT)/ Histone modifiers
demethylase ATP-dependent chromatin remodeling complexes
DNA demethylasesHistone acetyltransferase (HAT)/
Histone deacetylases (HDAC)
K-Ac
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Histone Coactivators
methyltransferase Scaffold
(HMT)/ Histone modifiers
demethylase ATP-dependent chromatin remodeling complexes
Me-K DNA demethylasesHistone acetyltransferase (HAT)/
Histone deacetylases (HDAC)
K-Ac
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Histone Coactivators
methyltransferase Scaffold
(HMT)/ Histone modifiers
demethylase ATP-dependent chromatin remodeling complexes
Me-K DNA demethylasesHistone acetyltransferase (HAT)/
Me-R Histone deacetylases (HDAC)
K-Ac
Active chromatin
Me-K
Me-R
K-Ac
Me-K
Me-R
K-Ac Histone Code
Me-K
Me-R
K-Ac Histone Code
Me-K
Me-R
K-Ac Histone Code
Me-K
Me-R
K-Ac Histone Code
Gene activation
Me-K
Me-R
K-Ac Histone Code
Gene repression
HP1
Me-K
Me-R
K-Ac Histone Code
Me-K
Me-R
K-Ac Histone Code
M HMT
Me-K
Me-R
K-Ac Histone Code
Me-K
Me-R
K-Ac Histone Code
Me-K
Me-R
K-Ac Histone Code
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Activators
Coactivators
Scaffold (HAT/HDAC)
Histone modifiers ex: histone acetylase/deacetylase
ATP-dependent chromatin remodeling complexes
DNA demethylases
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
Transcriptional Regulation in Eukaryotic Chromatin
Inactive chromatin
Core histones H2A, H2B, H3 and H4
DNA
Linker histone H1
Active chromatin
許志宏
Viral proteins
A common theme
Chromatin regulators
Viral proteins which regulate histone modifier activity
DNA Viruses
RNA Viruses
DNA Viruses
RNA Viruses
DNA Viruses
RNA Viruses
Virus-Cell Interaction
Could chromatin modifiers be the
common targets?
Inactive chromatin
RNA polymerases
Active chromatin
RNA
Alberts et al
Robert Roeder
Rockefeller University
hnRNA
snoRNAmicroRNA
resistant low h
10
igh
hnRNA
microRNA
Sequence of human CTD
Inactive chromatin
RNA polymerases
Alberts et al
TFIIA
activates TFIID
TBP by TAFII230
relieving the binds to TBP
repression at its DNA-
caused by binding
TAFII230 surface in
solution
TFIIF
contains TFIIB
RAP74 which provides
is an ATP- surface
dependent recognized
DNA helicase by RNAP
and can melt
TFIIE:allow
the DNA, and
RNAP to
RAP 38 which
move on
binds to
RNAP
Allow RNAP F20.11,
to bind to Gene VII
DNA
TFIIH
contains
ATPase,
helicase and
kinase
activities
Can
phosphorylate
CTD of RNAP
And relieve
RNAP from
promoter
F20.11,
Gene VII
Orphanides and Reinberg, 2002, Cell 108, 439
CTD
Inactive chromatin
Inactive chromatin
Repressors
Corepressors
Scaffold
Histone deacetylases RNAPII
ATP-CRC
DNA methylases
HeLa
Antibody Core
Dish Wash Core
Gene-specific transcriptional activation in eukaryotic chromatin
Inactive chromatin
Exchangeable subunits in
coactivator/corepressor complexes
Factor modifications: SUMO …
Repressors Activators (Tissue, Cell, developmental stage-specific)
Corepressors Coactivators
Scaffold Scaffold As a signal or code
Histone deacetylases Histone modifiers ex: acetyltransferases (HAT)
ATP-CRC ATP-dependent chromatin remodeling complexes
DNA methylases DNA demethylases?