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DEPARTMENT OF TECHNICAL EDUCATION

ANDHRA PRADESH
Name : A. Vinay Kumar
Designation : H.O. D
Branch : Applied Electronics and Instrumentation
Institute : Q.Q.GPT , HYD
Year/Semester: VI Sem
Subject : Analytical Instrumentation
Code No : AEI-603
Major Topic : Mass spectroscopy & Chromatography
Duration : 50Mts
Sub Topic : Introduction to chromatography
Teaching Aids : Animations, Diagrams

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Objectives

On completion of this topic you would be


able to know about Liquid Chromatography

• Introduction

• Block diagram

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Recap

In last class we have studied about gas


chromatograph
• Introduction

• Principle

• Operation

• Applications

• Detectors

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Introduction

• Chromatography is a physical method of separation of

the components of a mixture by distribution between two

phases

• One is a stationary phase of large area

• Other is a mobile phase which passes along the

stationary phase

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Definition

• If the mobile phase is liquid then the chromatography is

called “ Liquid Chromatography”

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High Pressure Liquid Chromatography
(HPLC)

• It can be considered as the oldest of all chromatographic


process

• High Pressure Liquid Chromatography (HPLC) is similar


to gas chromatography

• But the process is carried through in liquid form by a


solvent

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High Pressure Liquid Chromatography
(HPLC) …….

• The substance was not be vaporized

• HPLC can be used where the substance can not be

analyzed using gas chromatography

• The use of pressure increases the linear velocity

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High Pressure Liquid Chromatography
(HPLC) …..

• It gives the components to less time to diffuse within the

column

• Improves the resolution of the chromatogram

• It introduces a refinement called the Gradient elution

• i.e. variation of the mobile phase during the analysis

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Classification

Liquid Chromatography

Paper Column Thin layer

Liquid/Liquid Liquid/Solid Gel Ion


(Partition) Adsorption permeation
exchange

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Liquid chromatography

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Liquid chromatography

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Block diagram

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Description

The block diagram consists of


• Mobile phase system

• Pump programmer

• Sample valve

• Column

• Detector and recorder

• Data processing and printer

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Summary

In this class we have learnt about Liquid


Chromatography

 Introduction

 Block diagram

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Quiz

1. The mobile phase in Liquid Chromatography is

a) Gas

b) Solid

c) Liquid

d) None

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Frequently Asked Questions

1. Define Liquid Chromatography ?

2. Give the Classification of Liquid Chromatography

3. Give the block diagram of Liquid Chromatography

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