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Lysine decarboxylation
• Reaction in the butt
Lysine deamination
is an aerobic process which occurs on the slant of the
media
If the bacterium can deaminate Lysine in the slant, it will
produce an unknown orange product
This orange product will react with the Brom-cresol
Purple
If the organism has the ability to deaminate lysine, the
ammonia produced will react with the ferric ammonium
citrate to produce a dark red color on the slant of the
tube
(Positive deamination: red slant)
(Negative deamination: purple slant)
The genus that will give a positive red slant are
Proteus, Providencia, Citrobacter and Morganella
is an anaerobic process which occurs in the butt of the
media
Due to overneutralizing the acid formed from glucose
fermentation
The bacteria that is inoculated into the butt of this tube
can ferment the glucose, producing a low or acidic pH
This induces the bacteria to form the carboxylase
enzyme which removes the -COOH from the lysine
molecule, creating an amine end product, that is
alkaline in pH
If the organism has the ability to decarboxylate
lysine, it produces an amine end-product which reacts
with the pH indicator to give a purple color in the butt
of the tube
(Positive decarboxylation: purple butt)
(Negative decarboxylation: yellow butt)
Organisms which produce hydrogen sulfide gas will
exhibit a black precipitate in the butt of the tube
Stab the butt twice all the way down, then streak
the slant using a straight wire needle