Ogan Gurel, MD

Biology 301
Lecture # 7
Roosevelt University

Biology 301
Cellular and Molecular Biology
Spring 2002

Lecture # 7: DNA Replication

 Ogan Gurel, MD
Biology 301
Lecture # 7
Roosevelt University

Overall outline
 DNA as the genetic material
 DNA Replication
 DNA Repair
 DNA > RNA > Protein (revisited)
 Ogan Gurel, MD
Biology 301
Lecture # 7
Roosevelt University

DNA as the genetic material

 Transformation of bacteria
 Phage DNA entering a cell
 Hershey - Chase experiment
 The structure of a DNA strand
 Purines & Pyrimidines
 X-ray diffraction pattern of DNA
 The Double Helix
 Base pairing in DNA
 DNA Replication
 Ogan Gurel, MD
Biology 301
Lecture #7

Transformation of bacteria
Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

Phage DNA entering a cell

Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

Hershey - Chase experiment

Roosevelt University
The structure of a DNA Ogan Gurel, MD
Biology 301
Lecture #7

strand
Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

Purines & Pyrimidines

Roosevelt University
X-ray diffraction pattern of Ogan Gurel, MD
Biology 301
Lecture #7

DNA
Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

The Double Helix

Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

Base pairing in DNA

Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture # 7
Roosevelt University

DNA Replication
 A model for DNA replication: the basic concept
 Three models of DNA replication
 The Meselson-Stahl experiment tested three
hypotheses of DNA replication
 Replication begins at the “origin”
 Incorporation of a nucleotide into a DNA strand
 The two strands of DNA are anti-parallel
 Synthesis of leading & lagging strands
 Priming DNA synthesis
 The main proteins of DNA replication and their
functions
 A summary of DNA replication
 Ogan Gurel, MD

A model for DNA replication: Biology 301

Lecture #7
Roosevelt University

the basic concept

Three models of DNA Ogan Gurel, MD
Biology 301
Lecture #7

replication
Roosevelt University
The Meselson-Stahl experiment Ogan Gurel, MD

tested three hypotheses of DNA

Biology 301
Lecture #7
Roosevelt University

replication
 Ogan Gurel, MD

Replication begins at the Biology 301

Lecture #7
Roosevelt University

“origin”
 Ogan Gurel, MD

Incorporation of a nucleotide Biology 301

Lecture #7
Roosevelt University

into a DNA strand

 Ogan Gurel, MD

The two strands of DNA are Biology 301

Lecture #7
Roosevelt University

anti-parallel
 Ogan Gurel, MD

Synthesis of leading & lagging Biology 301

Lecture #7
Roosevelt University

strands
 Ogan Gurel, MD
Biology 301
Lecture #7

Priming DNA synthesis

Roosevelt University
 Ogan Gurel, MD

The main proteins of DNA Biology 301

Lecture #7
Roosevelt University

replication and their functions

A summary of DNA Ogan Gurel, MD
Biology 301
Lecture #7

replication
Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture # 7
Roosevelt University

DNA Repair
 Excision repair of DNA damage
 The end-replication problem
 Telomeres and telomerase
Excision repair of DNA Ogan Gurel, MD
Biology 301
Lecture #7

damage
Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

The end-replication problem

Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture #7

Telomeres and telomerase

Roosevelt University
 Ogan Gurel, MD
Biology 301
Lecture # 7
Roosevelt University

DNA > RNA > Protein (revisited)