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()
30 ;
1618 ,
24 .
- ()
,
(0,5 )
: 30
1.
2. ,
0,5
3.
4. 510 .
5. 16-18 33-35 (
)
6. :
S. aureus ATCC 25923 mecA ( 23-29 );
S. aureus ATCC 43300 mecA- ( 21 )
,
.
,
- .
1. Staphylococcus spp.
: ()
S. aureus and
S. lugdunensis
,
S. lugdunensis
,
30
,
(mecA+)
(mecA-)
21
22
24
25
30
ESBL
, ESBL, .
ESBL-
III-IV (8-16 /),
ESBL.
ESBL
-- .
-
, ESBL
III (, ),
,
/). 20-30 .
,
(/
/)
.
E. coli ATCC 25922;
K. pneumoniae ATCC 700603 (ESBL+)
- () (90 )
,
(0,5 )
1. .
2. ,
0,5 .
3.
.
4. 510
1.
5. 18-20 37.
6. .
MBL
--
(-) MBL .
P. aeruginosa ATCC 27853 (MBL-)
P. aeruginosa 565 (MBL+)
2.
, 15 ( )
, . 5(!) 0,5 M
.
CAZ
CTX FEP*
AMC
CAZ
CTX FEP*
1. ESBL.
CTX , 30 , CAZ , 30 , AMC / , 20/10
MEM
IPM
CAZ
2. MBL.
IPM , 10 ; MEM , 10 ; CAZ , 30
(-)
,
:
E. coli ATCC 25922
: 10 10 .
: -.
0,9% NaCl ( )
0,5 McFarland
1. E. coli ATCC 25922 ( ) 0,5
McFarland.
2. 1:10.
3. (E. coli ATCC 25922) ( - ).
( ) 3-10 .
4. () ( ):
90 (100) 1 .
150 4 (. 1, 2).
5.
(/ - ,
) ()
. 20-25 . (. 1, 2).
6. 35 16-20 .
7. :
E. coli ATCC 25922
. (. 1, 2).
E. coli ATCC 25922
. (. 1, 2).
. ,
.
; >90%
>90% KPC- .
-- .
.
Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing; TwentyThird Informational Supplement. CLSI document M100-S23. 2013. P.53-56
E. coli ATCC
25922
E. coli ATCC 25922. K.
pneumoniae ATCC BAA-1705,
, ,
.
E. coli ATCC 25922
.
1. 100 .
(1) K.pneumoniae ATCC BAA-1705,
(2) K.pneumoniae ATCC BAA-1706,
(3) ,
2. 150 .
(1) K.pneumoniae ATCC BAA-1705,
(2) K.pneumoniae ATCC BAA-1706,
(3-8) :
(3, 4, 5, 7, 8)
(6)
100 - B-PERII 1
100 .. 2
(, , .)
Rapid CARB Screen Kit:
- CARB ( + )
- CARB Negative Control