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680

: 1681000
1681001
1681002
1681003
-
125167 , -., 37, . 14
: (495)721-1404 : (495) 721-1412 E-mail: post@bio-rad.ru

2/43
1 ....................................................................................................................................... 3
2 ................................................................................................................. 3
2.1 ....................................................................................................... 3
2.2 ....................................................................................................................................... 4
2.3 ........................................................................................................................... 5
3 ......................................................................................... 6
3.1 ..................................................................................................................... 6
4 ....................................................................................................................... 7
4.1 ................................................................................................................................................... 7
........................................................................................................................................... 7
................................................................................................................................... 7
.......................................................................................... 8
................................................................................................................................................. 8
................................................................................................................................................... 8
. . .......................................................................... 8
................................................................................................................................................14
4.2 ............................................................15
4.3 680 .....................................................................16
4.3.1 ....................................................................................................................16
4.3.2 .......................................................................17
4.3.3 ................................................................................18
4.3.4 ....................................................................................................19
4.3.5 . ...........................................................................20
4.3.6 Checkmark...........................................................................23
4.3.7 . . .............................................................................24
4.3.8 . .................................................................................25
4.3.9 ............................................................................................................29
4.3.10 ..................................................................................31
4.3.11 ) ................................................................................................33
5. ...........................................................................................................35
5.1 . .....................................................................................35
5.2 . .....................................................................................................................................35
6. . .............................................................36
7. . 680 ............................................................38
7.1 . .................................................................................................................38
7.2 RS-232..................................................................................................40
7.3 680 ........................................................................................41

3/43

1
680 (Model 680 Microplate Reader)
96-
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680

RS-232.

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415, 450, 490 655.
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. (495)7211404
(495)7211412

4/43

2.2

1.

2.
(LCD)
3.

4.

5.
6.

7.
RS-232C
8.
9.
( )
10.

11.
12.
13.
14.

5/43

2.3

1. Main
2. Start/Stop
3. Paper Feed
4.

(Up Arrow)
5.

(Left Arrow)
6.

(Down Arrow)
7.

(Right Arrow)
8. Dot/Function
9. Enter
10. Ten Keys

11. Print
12. Edit
13. Memory
Recall





,
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;
; (
)
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0 / EMP :
5 / QC : QC
1 / SMP :
6 / CAL :
2 / BLK :
7 / CP :
3 / STD :
8 / CN :
4 / CO : .
9 / CW :

6/43

3
3.1
1. .
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2.
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7/43

4
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8/43



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1.
2.
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(00000)
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"---" nm.
1 day, 1 month, 00 year, 0 hour, 0 minute .
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3.500.
3.500,
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9/43

(2) (Abs.) - , .
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10/43

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11/43

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2. k * CPx
3. k * COx x
4. CNx / k
5. CPx / k
6. COx / k
7. k + CNx
8. k + CPx
9. k + COx
10. k * CNx + CPx
11. (CNx + CPx) / k
12. k1 * CNx + k2 * CPx
,

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12/43


10 , 0 9.


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10 , 0 9.


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(+), (-).

13/43

(6) (Curve fit)



.
10
:
1. 4 (4p logistic Rodbard)
2. 5 (5p logistic Rodbard)
3. 4 - (4p logistic Cook)
4. 5 - (5p logistic Cook)
5. 5 (5p Exponential)
6. (Sigmoid logistic)
7. (Quadratic)
8. (Linear)
9. (Cubic spline)
10. (Point to Point)

.

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,
.
,
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(8) (Difference) .

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(1)

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(2)

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(3)

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14/43

(4)
()

.
= (R2 R1) * k
:
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R2 = ,
k =
RS-232
680
RS-232,
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680 ,
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15/43

4.2 .
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16/43

4.3 680
4.3.1 .

680

13:05
24/03/06


13:05


.
.

24/03/06



: .
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ENTER

.
Change/
.
00000. (. ).


01:EndPoint Assay 01
M405(1)R605(6)
:999s,.
.:.
24/03/06
. .
01:Kinetic-1
M405(1)R605(6)30.
:999s,, .
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24/03/06

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24/03/06
Checkmark

. .

01:EndPoint Assay 01
M405(1)R605(6)
:999s,.
38.2/40.2 24/03/06

M: .
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(//)
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M: .
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38.2/40.2
24/03/06

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17/43

4.3.2 .



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18/43

1:400nm 5:650nm
2:450nm 6: . . . nm
3:475nm 7: . . . nm
4:650nm 8: . . . nm


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4.3.3 .


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19/43

4.3.4



..

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Memory Recall .



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#01
*End Point #01
! ABC assay
End Point-03



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#01
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! GALT Assay

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..

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..
01 20/05/00 14:00
02*21/05/00 17:00
03 20/05/00 18:00
? 04 20/05/00 19:00

01* 20/05/00 09:00


02 20/05/00 10:00
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20 / 05 / 01 09:00

Checkmark .

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20/43

4.3.5 .



Edit .



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- .


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= 20 (2-30)

= 25 sec (0-999)
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= 20 (2-30)

= 25 sec (0-999)



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: 200 sec

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2->3
3->4
4->5

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100sec
100sec
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21/43


:
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: 655 nm




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: .
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(25 - 45)

22/43

.

. .: 0.910
: 10 - 20


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),
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= (R2R1)*k
k = 999.999
R1: 1
R2: 2


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[ABC]


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23/43

4.3.6 Checkmark


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No: 0016
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.

405nm
S1: 0.153
S2: 0.301
.
S3: 1.342
S4: 2.190

. CV%
.CV% S1: 10%

S2: 05%
.
S3: 02%
S4: 02%


405nm: 1.886 1.543
450nm: 1.171 0.958
492nm: 0.599 0.490

540nm: 0.330 0.244
620nm: 0.180 0.120
690nm: 0.103 0.062

"" ""
S1 S4.
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.

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. : 405nm, 450nm, 492nm, 540nm, 620nm,
690nm. Enter
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S1 S4.
. CV%. Enter
.


, .

. , 405nm, 450nm 492nm.
"", "" ""
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, , 540nm, 620nm
690nm. Enter .

24/43

4.3.7 ..



Edit .

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End point.

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[ . ]

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= 2.000
= 0.000
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3.500. Enter
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25/43

Kit name
. #01
End point #01

[ABC]

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4.3.8 .
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3.
4.
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. >= 9999
: 50%
: mol/l

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. Enter

.

26/43

- .


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. < 1111
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.
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.
.
:

"CNx"

.

- .

"CPx"

.

.

.

27/43

c. Cutoff Formula setting



[ k * CNx ]
k * CPx
k * COx
CNx / k
CPx / k
COx / k
k + CNx

.

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Enter
.
:
1. k * CNx
2. k * CPx
3. k * COx x
4. CNx / k
5. CPx / k
6. COx / k
7. k + CNx
8. k + CPx
9. k + COx
10. k * CNx + CPx
11. (CNx + CPx) / k
12. k1 * CNx + k2 * CPx
.

k + CPx
k + COx
k * CNx + CPx
(CNx + CPx) / k


k = 0.01 (0.01-10)
: 50%

k1 * CNx + k2 * CPx


k1 = 0.01 (0.01-10)
k2 = 10.0 (0.01-10)
: 50%

. #1 to #4
k

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k1 k2


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:
"CNx"
(absorbance value)

. "CPx"
(absorbance value)

.

. .


.

28/43

d. .

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.

: 9999
: mol/l

01: mol/l
02: mmol/l
03: [ umol/l ]
04: nmol/l

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: 50%
: mol/l

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(Absorbance) .

29/43

4.3.9 ( ).
()

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.


= 05 (0, 212)

STD # 1: 9.999
STD # 2: 9.999
STD # 3: 9.999
STD # 4: 9.999

01: mol/l
02: mmol/l
03: [ umol/l ]
04: nmol/

0
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.



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17
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: .
1. mol/l
11. pg/ml
2. m mol/l
12. U/ml
3. u mol/l
13. IU/ml
4. n mol/l
14. uIU/ml
5. p mol/l
15. mIU/ml
6. mg/l
16. EU
7. ng/l
17. Arbit
8. pg/l
9. ug/dl
10. ng/ml

30/43
b.


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.


10
, .
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].
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.
:
1. 4 (4p logistic Rodbard)
2. 5 (5p logistic Rodbard)
3. 4 - (4p logistic Cook)
4. 5 - (5p logistic Cook)
5. 5 (5p Exponential)
6. (Sigmoid logistic)
7. (Quadratic)
8. (Linear)
9. (Cubic spline)
10. (Point to Point)

X- , Y-
X- , Y-
X- , Y-
[ X- , Y- ]


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.
:
1.
2.
3.
4.

31/43

4.3.10 .

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a.

:
: 400 nm
: 655 nm
:
: 400 nm


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: .
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10 / ,
15 / ,
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32/43

: .
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4.3.11 .
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a.

N 1 2
3 4
A B CN1 S01 S01
B B CN1 S02 S02
C B CN2 S03 S03
[F] 5
6 7
8
A X11 X12 X13 X14
B X21 X22 X23 X24
C X31 X32 X33[X34]


. 10 .

:


1. Blank():
B
2. Sample():
X00X99
3. Standard():
S01S99
4. In-kit Control: Positive
CP0CP9
Control( )
5. Weak Positive Control (
CW0CW9
)
6. Negative Control (
CN0CN9
)
7. Cutoff Control ( . )
CO0CO9
8. Calibrator ()
CL0CL9
9. QC Control( ):
QC0QC9
10. Empty well( ):
:
0/EMP - Empty well( )
1/SMP - Sample well()
2/BLK - Blank well()
3/STD - Standard well()
4/CO - Cutoff Control well ( . )
5/QC - QC well( )
6/CAL - Calibrator well()
7/CP - Positive Control well( )
8/CN - Negative Control well( )
9/CW - Weak Positive Control well ( )

:

:

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[F],
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[S01] () ,
S01 - ,
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34/43

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2. Sample:
00-99
3. Standard: 01-99
4. Control
Calibrator:
0-9
b.

,


.
.

35/43

5. .
! : .
, .
! : . .
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1. , ,
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2.
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3. ,
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2. ,
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5.
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6.
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7. .
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36/43

6. .
680
( LCD
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1.

2.

a.

.
b.

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c. .


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STOP.
,
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37/43

5.

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6.


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7.

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8.

9.
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(. 5.1).

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38/43

7. . 680
7.1. .




, 4
, 11 7 .
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(CENTRONIX)
5/7 , , , 112
- ,
- ,
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- V ()
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10 ENDPOINT
230 KINETIC
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3000
400 750
- 10 ,
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450, 415, 490,655
405, 540, 550, 570, 595, 630, 750

- 6
- 10

39/43

(ACCURACY)

(REPRODUCIBILITY)

()

15
30

0.000 - 3.500 OD
0.001OD
1.0 % 0.010 0 3.0 OD / 490
1.0 % 0 2.0 OD
2.0 % 0 3.0 OD
1.0 % 0.005OD 0 - 2.0OD
1.5 % 0 - 3.0OD
<1.5 % 0.005OD 0 - 3.0OD
0.01OD 1.0OD
.
25 - 45 0.1 ( 5
)
< 0.5 37 . 25.5
< 0.7 37 . 25.5
< 5
- 100-240 ,
- 47-63
100 .
340/330/152 (//)
5.5
5 35
0-95%
-20 500

40/43

7.2 RS-232.
,
.
1. (SIGNALS).
TXD: transmit data
RXD: receive data
DTR: data terminal ready
DSR: data set ready
CTS: clear to send
RTS: ready to send
SIGNAL GROUND (SHIELD GROUND).
2. (TRANSMISSION)
()
9600
() 8

1


3. (DATA)
ASCII.
(
ASCII).

.
4.

41/43

7.3 680
: < ><><>[<><
>]< >
: - "EIA. READER" 680
1. <>: "ID" - ID , Model 680 reader;
<> "ERE" <>< ><><1A>< >
<1D>: "0680" ID Model 680 reader.
2. <>: "AQ" -
; .
, .
Remote Control Mode ( )
.
(release - RL)
STOP () .
<> "ERE" <>< >< >
3. <>: "RL"
;
<> "ERE" <>< >< >
4. <>: "RS" -
;
<> "ERE" <>< >< >
5. <>: "MR" - ;
<>
"ERE"
<>< ><
><>< ()>
;
"On/Off:" (/) <#/><>: .
"Hours:" () <#><> : ,
.
"Plates" () <#><>: .
: <#/>, <#>, <#> - 4
ASCII. .
6. <>: ""- ;

<> "ERE" <>< >< >


7. <>: "RWELL" - ; <1>, <rw>,
<s/d>
< > 1 - , (1-12) ASCII
rw - , (1-8) ASCII
wpl - , (1-4) ASCII

42/43

wp2 - , (1-4) ASCII


(, )
<>
"ERE" <>< ><>< .
1><><. . 2 ><
>
< . 1> (
ASCII), "1.234"
< . 2> (
ASCII), " 1.234"
8<>: "RPLATE" - ; <mix>, <wpl>, <wp2>
< > mix- ( );
(0-9) ASCII
wpl , (1-4) ASCII
wp2 - , (1-4) ASCII
(, )
<> "R"<>< ><>< .><>
9. <>: "RL"- ;

<> "R"<>< ><>< .><>

< .>:
<><. >< ><>
<><. >< ><><
><>
.
<>: "BIO-RAD Model 680 reader "<>
<. >: "Mes. filter: "<wpl><BK>
"Ref. filter: "<wp2><BK> -
< .> ".begin"<BK><8 12
>< ><>:".end"<>

ASCII.
. 1=(1-12), 2=(1-12), 8=(1-12).
< >
".begin"<BK><8 12
>< ><>:".nd"<>

ASCII. .
1=(1-12), 2=(1-12), 8=(1-12).
< >. 2-
(0-255) ASCII ASCII,
.
".begin"<BK> .

43/43

< .> < >:


".begin"<>
" 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 0.112"<cr> " 0.201
0.202 0.203 0.204 0.205 0.206 0.207 0.208 0.209 0.210 0.211 0.212"<cr> " 0.301 0.302
0.303 0.304 0.305 0.306 0.307 0.308 0.309 0.310 0.311 0.312"<cr> " 0.401 0.402 0.403
0.404 0.405 0.406 0.407 0.408 0.409 0.410 0.411 0.412"<cr> " 0.501 0.502 0.503 0.504
0.505 0.506 0.507 0.508 0.509 0.510 0.511 0.512"<cr> " 0.601 0.602 0.603 0.604 0.605
0.606 0.607 0.608 0.609 0.610 0.611 0.612"<cr> " 0.701 0.702 0.703 0.704 0.705 0.706
0.707 0.708 0.709 0.710 0.711 0.712"<cr> " 0.801 0.802 0.803 0.804 0.805 0.806 0.807
0.808 0.809 0.810 0.811 0.812"<cr> <checksum><cr> ".end"<cr>
: , , 3000
*.
:
"0000" -
"8071" -
"8072" -
"8073" -
"8074" -
"8075" -
"8076" -
"8077" -
"8078" -
"8079" -
"8080" -
"8081" -
: 680 "Q",
,
"8073" -
.
"AQ",
,
"START/STOP" ("/") "RL".