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Культура Документы
( 4.7.2, 2008)
Carl Zeiss
Imaging Associates Ltd.
www.imas.co.uk
,
AxioVision. .
AxioVision
; ,
. .
, .
AxioVision ,
, , , AxioVision, 4.6
.
AxioCam.
, , .
, ,
.
, ,
Carl Zeiss.
1 AxioVision............................................................................5
............................................................................................................... 7
2 AxioCam.........................................................8
2.1 ............................................................................................................................... 8
......................................................................................................................... 10
....................................................................................................................... 10
( )..............................................12
................................................................................................... 12
2.2 AxioCam..................................................................................................... 13
..............................................................................................13
.................................................................................................................................. 13
...................................................................................13
.................................................................................................14
.......................................................................................................... 14
.................................................................................................... 15
................................................................................................................. 15
3 .15
4 ...........................................................................18
......................................................................................................... 18
...............................................................................18
.......................................................................................20
.............................................................................22
5 , ....................................................................23
6 .........................................................................25
...........................................................................27
..................................................................................27
7 .............................................................................................................................. 28
8 .............................................................29
9 ........................................................................................... 32
10 ..................................................................................................................... 33
12 ().................................................38
12.1 .....................................................................................40
12.2 Z-............................................................................................................................. 42
12.3 ........................................................................................................ 44
12.4 ......................................................................................................... 45
13 ...............................45
14 ........................................49
15 ............................................................................................................................. 50
1 AxioVision
AxioVision ,
. AxioVision ,
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. 8 ,
.
. AxioVision
AxioVision
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Microsoft Windows Carl Zeiss Vision
.
Toolbar
Document area
(Images, Forms,
Reports)
Workflow
Workarea
(, ,
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5
AxioVision , , Options
() Tools () Reset Window Layout ( ),
AxioVision.
(MTB2004): AxioVision
ZEISS. ,
, AxioVision
MTB2004.
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other ().
: MTB2004 Simulate Hardware (
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AxioVision, :
Toolbar ( ): , ,
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Workflow ( ): ,
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Workarea ( ):
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Document area ( ):
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AxioVision - .
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Multichannel ( ): ,
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Z-stack (Z-): , .
Time lapse ( ): ,
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MosaiX: ,
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Mark&Find: ,
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12.
2
AxioCam
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AxioVision, ''
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AxioVision ,
FireWire. ,
Carl Zeiss, Carl Zeiss.
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TWAIN,
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TWAIN AxioVision .
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Acquisition ()
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AxioCam .
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FireWire, , , AxioCam HRc HRm Rev. 1, 2 AxioCam
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2.1
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AxioCamHR.
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Measure (),
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AxioCam .
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Frame
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Close after Snap
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2.2 AxioCam
Frame .
AxioCam , ,
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(2x2, 3x3 ..) .
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General ()
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General.
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Enable,
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Processing ().
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: Carl Zeiss
AxioCam ICc.
3
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Open Image ( ) File (). ,
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4
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Zoom In / Zoom Out ( / ) ( F7 F8).
Auto Zoom ( ):
.
Normal View ( ):
( ),
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Full Screen ( ): ESC,
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Navigator ().
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Navigator, ,
.
.
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, AxioCam.
, .
Properties (), Properties, Display
().
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(RGB).
,
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Linear () .
Min / Max ( / ),
.
: Best Fit ( ) Min / Max,
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. 5 %,
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Properties, .
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12 .
View
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Extended Settings ( ),
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Z-, Create Z-Stack image
( Z-).
,
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Create Time
lapse image (
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,
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Gallery ()
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DAPI, FITC TRTIC
, .
Channel Color (
),
,
DAPI
.
Z-,
Z-Stack,
z.
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Shift
.
,
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Ctrl ,
,
.
( 14).
,
.
,
Ctrl
. Z, ,
z, , Shift
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z.
z. Extract
Selections ( ),
.
Create Image (
)
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5 ,
ZVI. ,
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.
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ZVI.
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Save As ( ) File () . ,
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ZVI .
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, ( , ), Export
() File. ,
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(Base name)
(Save in)
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folder ( ),
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Mode ()
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z .
Generate merged images (
)
.
File Type ( )
.
Convert to 8 bit ( 8-) (
*.tif),
8 TIFF.
Gray Scale ( )
-.
Apply display mappings (
)
( 4).
Burn in Annotations ( )
.
Start (), .
: , File, Export
Batch (). Add files type ( ),
, , Run batch
( ).
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AxioVision , 3,
Open Image ( ) File.
ZVI.
( Z-), 13.
6
AxioVision. -,
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AxioVision
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Scaling () (Workarea).
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.
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(
1300x1000 ).
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Advanced (), New
(), .
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Single distance
( ).
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: .
, ( x y).
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( ).
,
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,
(
9).
: x y ,
,
.
.
*
Properties
() Attributes ().
Scale bar (
)
.
,
.
:
optovar / tube, , ,
optovar
()
AxioVision MTB2004
.
MTB2004 , AxioVision ,
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.
.
,
"" ,
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, Measure
() Menu ().
7
,
. ,
Annotations () (
Annotations () 8).
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.
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.
,
,
Properties () Attributes () (
6: ).
: Undo () Redo
() Edit (),
.
: ZVI,
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5.
Frequent Annotations ( ):
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8
AxioVision, ,
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.
( ),
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AxioVision , ,
, Microsoft Windows.
, AxioVision ,
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.
,
,
Workflow.
, 9
.
:
.
Keyboard () My
AxioVision.
.
Tools Options,
Naming ( ).
.
(, 2 = 01 99)
FormatID ( ), %U
Microsoft Windows.
Tools Options,
Storage
().
Autosave
() (
Microsoft Windows,
).
Tools Options,
Acquisition ().
.
Auto save (),
, Storage (),
, Naming (
).
Carl Zeiss Vision Image (*.zvi),
.
Snap (),
.
9
,
. , . ,
, , , CDROM, ZIP . ZVI,
.
, ,
, ,
.
AxioVision ,
My Documents
. ,
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Microsoft Windows
My Documents All Users ( ),
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Tools Options, Folders
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(
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(
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Folders info ( ).
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Import / Export
Configuration ( /
) Tools.
Export ()
.
Next ().
.
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, Import
() .
: (Save to Zip file
( ))
. ,
.
10
Carl Zeiss
, AxioVision,
, .
, ,
.
:
Microscope ()
.
Microscope
Microscope
.
,
.
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:
Settings
Editor ( )
Tools.
FITC.
New (),
.
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Edit
()
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TRITC,
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.
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( ) .
, Apply () ( Tools, Settings,
User (, , )), .
.
( 8) .
11
AxioVision.
, Measure
(). .
.
Interactive Measurement (
) Measure,
Configure Tools ( ...)
AxioVision
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.
Configure Tools
.
,
, Draw Features ( )
.
,
.
AxioVision
, Outline spline (
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Outline spline
, .
OK, .
.
,
Properties (), Measurement (),
.
(, Excel).
.
Area
Center X
Center Y
Perim
BoundWidth
BoundHeight
Diameter
Radius
DensMeanR
DensMeanG
DensMeanB
DensStrR
DensStrG
DensStrB
.
, ,
Properties (),
Measurement ().
12
()
AxioVision - ,
.
:
: ,
, 32 . ,
( -) .
Z-: .
.
,
,
Z-.
: , (Inside4D)
, AxioVision,
.
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.
,
,
.
ZVI ( 5),
: ,
Mark&Find.
.
.
Multidimentional Acquisition (
) Workarea;
, .
Experiment ()
, C, Z, T
(Multichannel), Z (Z-Stack) (Time Lapse) .
.
Experiment_1, Experiment_2
.
,
, ,
(Multichannel), Z- (Z-Stack)
(Time Lapse).
12.1
,
; ,
Z- (Z-Stack)
(Time Lapse).
C (),
.
: ,
.
,
(1).
DAPI .
,
.
DAPI, .
, My_DAPI.
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, DAPI,
DAPI_Narrow DAPI_Wide.
: , AxioVision
, ,
.
(Exposure)
.
:
(Auto) .
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,
,
.
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.
Z-,
.
(Fixed) Time (). ,
, Measurement ().
(Camera) ,
( 2),
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,
( 10). ,
, ,
DAPI , ,
, , .
(Autofocus),
.
.
: Go (),
.
, (Save to pool),
.
Duplicate (), My_DAPI.
, , FITC.
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. ,
.
, ,
. Extended Parameters ( ),
Extended Channel Parameters ( ),
.
My_DAPI,
. ,
,
.
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, Save to pool (
),
.
Channel pool ( ),
,
Add to Experiment (
).
,
,
.
Experiment ().
Save () ,
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Load ().
: ReUse ( )
, .
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.
Hardware Settings (
)
, , Start (),
.
, (,
My_DAPI), DAPI Snap
().
.
:
.
,
.
,
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Z-.
,
Z-.
Z
.
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3, . ,
.
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12.2 Z-
Z, Z-.
:
.
;
Center ().
;
.
Center,
.
,
, .
15 0,5 . ,
8, 7 7
.
Z-Stack navigation
( Z-) ,
.
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.
Start / Stop ( / ).
,
Start () Stop ().
' ', ' ',
,
,
.
: Z-,
AxioVision z 1,
z 2 . ,
z. All Channels per Slice
( ), AxioVision z,
z, .
z, .
Optimal distance ( )
, z , .
(Nyquist criteria),
, .
.
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AxioVision , .
.
, ,
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AxioVision Deconvolution (
AxioVision) ,
.
" "
, .
12.3
T, .
: .
, ,
10 , 15 .
135
( t=0).
Cycles (),
,
.
:
.
, Z-
, ,
, ,
.
" "
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12.4
Experiment ()
.
.
;
Z- , ,
Center () Start / Stop ( / ) .
Z-
, .
.
,
.
, ,
,
.
13
AxioVision.
,
5, Open ()
File () ( Open Image ( )).
.
File Import to ZVI ( ZVI).
, Z- .
1:
(Setup Image Dimensions)
,
( , Z-
).
. Scale
lateral (X) ( (X))
/ .
,
y, ,
Different Y scaling (
Y).
/ ,
,
.
Next ().
2: (Channel Setup)
8 ,
, .
Add channel ( ) Next
.
3: Z- (Z-Stack parameters)
.
Z-.
Extended parameters (
), ,
. ,
,
, ,
, .
4: T (T Parameter Setup)
,
.
5: (Compose Images) ,
.
. , Get Selected
( ). .
Consistency (), ,
. ,
, Preview ( ).
Finish () , :
, ZVI Save
() ( Save as ( )) File ().
14
, Z-
,
(, Z-
). (Image Editor) Edit ()
Change Image Dimensions ( )
Change Image Dimensions (
).
( 4).
1: (Select action) ,
(Keep)
(Remove), .
-
2: (Channel Selection)
,
.
3: (Reduce
pixel resolution)
,
.
X
.
4: Z- (Z-Stack selection)
Z-.
, Enable
this action ( ).
z, From
() To () ,
, Get player
position ( ),
() .
""
(,
/).
5: (Time Point Selection)
. , , Enable this action
( ). Z-.
Preview ( ) / Finish ().
Save () (
Save as ( )) File ().
15
, .
(, ),
.
,
.
, 8
= 256 , 12 = 4096 , 14 = 16384
.
,
.
,
.
Microsoft Windows,
.
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( Y)
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G, B,
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