28/11/2022 Page 1

UNAM
File: Caf-3 Date Run: 11-24-2022 (Time Run: 15:07:10)
Sample:
Instrument: JEOL GCmate
Inlet: My Inlet Ionization mode: EI+

30000000

24,000,000

18,000,000 21.42

12,000,000

6,000,000

TIC
Scan 400 600 800 1000 1200 1400 1600 1800
Min. 10 15 20 25 30
RIC - TIC
21.42

Min. 21.4

Integration Range 21.35 - 21.47 Total Area 105,713,969

Baseline at 21.35 Background 39,131,295
Exact RT 21.448 Peak Area 66,582,674

Peak Table

Peak Compound RIC Scan Baseline Total Background Peak

Name Range at Area Area

21.42 TIC 1056-1063 1056-1056 105713969 39131295 66582674

 28/11/2022 Page 2
UNAM
File: Caf-3 Date Run: 11-24-2022 (Time Run: 15:07:10)
Sample:
Instrument: JEOL GCmate
Inlet: My Inlet Ionization mode: EI+

Scan: 1060 R.T.: 21.42

Base: m/z 194; 99.9%FS TIC: 17039676 #Ions: 131

194
100
109
%50 55 67
28 137 165
0
m/z 50 100 150 200 250 300 350 400 450 500

Threshold: 2% of Base Displayed TIC: 17039676

Mass %Base Mass %Base Mass %Base Mass %Base Mass %Base Mass %Base Mass %Base
27.9912 4.5 51.9471 2.2 55.9502 4.6 69.8865 3.1 83.3896 2.1 108.8703 60.9 137.7926 2.3
39.9729 4.9 52.9498 3.3 65.9158 3.9 80.8963 5.2 93.8640 3.2 109.8527 7.8 164.7394 5.4
40.9821 5.2 53.9619 3.6 66.9267 31.8 81.9123 23.9 96.8697 2.3 135.7824 5.6 192.6548 10.6
41.9853 11.4 54.9728 37.6 67.9219 4.3 82.9087 2.5 107.8626 2.7 136.7773 6.1 193.6385 100.0

Mass %Base Mass %Base Mass %Base Mass %Base Mass %Base Mass %Base Mass %Base
194.6839 11.9

Threshold: 2% of Base Displayed TIC: 48040

NIST MS 1 of 1 (58-08-2)
Base: m/z 194 #Ions: 92 Mass %Base Mass %Base Mass %Base Mass %Base
Caffeine 15.0000 3.8 55.0000 43.9 82.0000 32.8 137.0000 6.2
100
194 28.0000 3.7 56.0000 7.9 83.0000 3.6 138.0000 2.9
O 40.0000 6.5 66.0000 6.2 94.0000 4.5 165.0000 7.7
N
41.0000 6.2 67.0000 43.8 97.0000 2.2 193.0000 13.5
N 42.0000 13.8 68.0000 5.2 108.0000 4.1 194.0000 100.0
109

O N N 52.0000 3.7 70.0000 4.1 109.0000 72.2 195.0000 10.3

53.0000 5.2 80.0000 2.4 110.0000 9.2
%
55 54.0000 6.8 81.0000 8.1 136.0000 6.0
82

42
137 165

0
m/z 50 100 150 200 250 300 350 400 450 500