EJERCICIO DE CINÉTICA ENZIMÁTICA

mmol/L-1 t=60 t=120 t=180 t=240 t=300 mmol/L-1 t=0 t=60 t=120 t=180 t=240 t=300
5 161 320 482 598 662 0.2 0 9600000 19200000 28920000 35880000 39720000
6 194 388 581 745 796 0.16666667 0 11640000 23280000 34860000 44700000 47760000
10 263 525 789 998 1120 0.1 0 15780000 31500000 47340000 59880000 67200000
20 400 798 1200 1520 1760 0.05 0 24000000 47880000 72000000 91200000 105600000
30 570 1150 1730 2470 2460 0.03333333 0 34200000 69000000 103800000 148200000 147600000

mmol/L-1 1/V0 1/V0 1/V0 1/V0 1/V0

0.2 1.041667E-07 5.208333E-08 3.457815E-08 2.78707E-08 2.51762E-08 Diagrama de Lineweaver-Burk
0.1666666667 8.591065E-08 4.295533E-08 2.868617E-08 2.23714E-08 2.0938E-08 0.00000012
0.1 6.337136E-08 3.174603E-08 2.112379E-08 1.67001E-08 1.4881E-08
0.05 4.166667E-08 2.088555E-08 1.388889E-08 1.09649E-08 9.4697E-09 0.0000001 f(x) = 4.24524320957274E-07 x + 1.81733463913375E-08
0.0333333333 2.923977E-08 1.449275E-08 9.633911E-09 6.74764E-09 6.77507E-09

0.00000008

0.00000006

f(x) = 2.12549883771174E-07 x + 9.05211125804512E-09

0.00000004
f(x) = 1.41434614849647E-07 x + 6.02437346955347E-09
f(x)
f(x) == 1.1651445595919E-07
1.06082033928843E-07x x++4.11434226556747E-09
3.77897110529956E-09
0.00000002
Vmax Km
50000000 20
0
111111111.11 22.222222222 0.02 0.04 0.06 0.08 0.1 0.12 0.14 0.16 0.18 0.2 0.22
166666666.67 16.666666667
1/V0 Linear (1/V0) Linear (1/V0) 1/V0
250000000 25
Linear (1/V0) 1/V0 Linear (1/V0) 1/V0
250000000 25 Linear (1/V0) 1/V0 Linear (1/V0)
PROMEDIO 165555555.56 21.777777778