S(g/L) V(g/L*min) 1/s 1/V S/V V/S

13.5 0.0942 0.07407407 10.6157113 143.312102 0.00697778

22.6 0.1661 0.04424779 6.0204696 136.062613 0.00734956
33.1 0.2201 0.03021148 4.54338937 150.386188 0.00664955
43.2 0.2427 0.02314815 4.12031314 177.997528 0.00561806
60.5 0.2909 0.01652893 3.43760743 207.975249 0.00480826
97.7 0.4388 0.01023541 2.27894257 222.652689 0.0044913
192.9 0.3135 0.00518403 3.18979266 615.311005 0.00162519
360.9 0.242 0.00277085 4.1322314 1491.32231 0.00067055
489.5 0.2238 0.0020429 4.46827525 2187.22073 0.0004572
603 0.1698 0.00165837 5.88928151 3551.23675 0.00028159
750.6 0.1364 0.00133227 7.3313783 5502.93255 0.00018172

Vmax:?
Km:?
Langmuir S(g/L) S/V
13.5 143.312102 S/V vs S
22.6 136.062613 6000
33.1 150.386188
5000
43.2 177.997528
4000 f(x) = 6.48054654242792 x − 263.677107343362
60.5 207.975249 R² = 0.93422698624258
97.7 222.652689 3000

S/V
192.9 615.311005
2000
360.9 1491.32231
489.5 2187.22073 1000
603 3551.23675 0
750.6 5502.93255 0 100 200 300 400 500 600 700 800
S

Lineweaver- Burk
1/V vs 1/S
12

10

8 f(x) = 65.7557994513995 x + 3.82948762281843

R² = 0.421605979096621
6
1/V

0
0 0.01 0.02 0.03 0.04 0.05 0.06 0.07 0.08
1/S

Eadie-Hofstee
V(g/L*min) V/S
0.0942 0.00697778 V/S vs V
0.1661 0.00734956 0.008
0.2201 0.00664955 0.007
0.2427 0.00561806 0.006
0.2909 0.00480826
0.005
0.4388 0.0044913
0.004
V/S

0.3135 0.00162519 f(x) = − 0.00115649595411714 x + 0.00382239004891291

0.003
0.242 0.00067055 R² = 0.00139175977428618
0.002
0.2238 0.0004572
0.001
0
0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5
V
 0.004

V/S
0.003 f(x) = − 0.00115649595411714 x + 0.00382239004891291
R² = 0.00139175977428618
0.002

0.1698 0.00028159 0.001

0.1364 0.00018172 0
0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5
V
3362
Vmax 0.15430908 (g/L*min)
km 40.6882185 g/L

0 600 700 800

Vmax 0.2611307 (g/L*min)

km 17.17091 g/L

5 0.06 0.07 0.08

Vmax 0.0038 (g/L*min)

Km 0.0012 g/L

382239004891291

0.35 0.4 0.45 0.5

382239004891291

0.35 0.4 0.45 0.5

Concent de lactosa (mol l-1 *102) Vel inicial de rxn (mol l-1 *103) S V 1/S
2.5 1.94 250 1940 0.004
2.27 1.91 227 1910 0.00440529
1.84 1.85 184 1850 0.00543478
1.35 1.8 135 1800 0.00740741
1.25 1.78 125 1780 0.008
0.73 1.46 73 1460 0.01369863
0.46 1.17 46 1170 0.02173913
0.204 0.779 20.4 779 0.04901961
 1/V S/V V/S Langmuir S S/V
0.00051546 0.12886598 7.76 250 0.12886598
0.00052356 0.11884817 8.41409692 227 0.11884817
0.00054054 0.09945946 10.0543478 184 0.09945946
0.00055556 0.075 13.3333333 135 0.075
0.0005618 0.07022472 14.24 125 0.07022472
0.00068493 0.05 20 73 0.05
0.0008547 0.03931624 25.4347826 46 0.03931624
0.0012837 0.02618742 38.1862745 20.4 0.02618742

Lineweaver- Burk

Eadie-Hofstee
V V/S
1940 7.76
1910 8.41409692
1850 10.0543478
1800 13.3333333
1780 14.24
1460 20
1170 25.4347826
779 38.1862745
 S/V vs S
0.14
0.12 f(x) = 0.000444819725688048 x + 0.0170268934308576
R² = 0.998440034202179 Vmax
0.1
0.08 km
S/V

0.06
0.04
0.02
0
0 50 100 150 200 250 300
S

1/V vs 1/S
0.0014
0.0012 f(x) = 0.0173803035334225 x + 0.000443002833687286
R² = 0.995966692207759
0.001 Vmax
0.0008 km
1/V

0.0006
0.0004
0.0002
0
0 0.01 0.02 0.03 0.04 0.05 0.06
1/S

V/S vs S
45
40 Vmax
35 f(x) = − 0.0246738319705166 x + 56.3136361335131 Km
30 R² = 0.983854666844126
25
V/S

20
15
10
5
0
600 800 1000 1200 1400 1600 1800 2000 2200
V
10
5
0
600 800 1000 1200 1400 1600 1800 2000 2200
V
 2500 (g/L*min)
42.5 g/L

2500 (g/L*min)
43.5 g/L

56.314 (g/L*min)
0.0247 g/L